Oxidative enzymatic alkene cleavage: Indications for a nonclassical enzyme mechanism

Lara, M.; Mutti, F.G.; Glueck, S.M.; Kroutil, W.;

J. Am. Chem. Soc., 2009, 131, 5368 – 5369. DOI: 10.1021/ja8097096


An enzyme preparation of Trametes hirsuta cleaves alkenes following neither the classical dioxygenase mechanism nor via a monooxygenase mechanism. A catalytic cycle for an alternative enzymatic alkene cleavage was proposed, whereby two oxygen atoms derived from two different oxygen molecules are incorporated into the product(s).