Asymmetric biocatalytic amination of ketones at the expense of ammonia and molecular hydrogen

Holzer, A.K.; Hiebler, K.; Mutti, F.G.; Simon, R.C.; Lauterbach, L.; Lenz, O.; Kroutil, W.;

Org. Lett., 2015, 17, 2431-2433. DOI: 10.1021/acs.orglett.5b01154


A biocatalytic system is presented for the stereoselective amination of ketones at the expense of NH3 and molecular hydrogen. By using a NAD+-reducing hydrogenase, an alanine dehydrogenase, and a suitable ω-transaminase, the R- as well as the S-enantiomer of various amines could be prepared with up to >99% ee and 98% conversion.