April 2021: HIMS-Biocat group contributes to “Biocatalysis for Practitioners” book
Our group has contributed with a book chapter entitled “Enzymes Applied to the Synthesis of Amines” to the book entitled “Biocatalysis for Practitioners — Techniques, Reactions and Applications” edited by prof. Gonzalo de Gonzalo and prof. Iván Lavandera and published by Wiley.
Book chapter by HIMS-Biocat group
CHAPTER 6, Enzymes Applied to the Synthesis of Amines in Biocatalysis for Practitioners. Techniques, Reactions and Applications (Ed. de Gonzalo, G.; Lavandera, I.), Wiley, 2021, pages 143–180.
By Francesco G. Mutti and Tanja Knaus
A great variety of biocatalytic methods are available for the synthesis of amines, in particular α-chiral amines. Therefore, chapter 6 aims to orient non-specialists to the available biocatalytic methodologies for the synthesis of α-chiral amines with a focus on the enzymes’ substrate scope and selectivity as well as the structural diversity of the obtained products. The presented enzymatic methods comprise the use of hydrolases, amine oxidases, transaminases, amine dehydrogenases, imine reductases, ammonia lyases, Pictet-Spenglerases and engineered cytochromes P450. Classification and reactivity, biocatalytic applications and selected important examples are discussed for each enzyme category. Methodologies entail the kinetic resolution or deracemisation of racemic amines and the asymmetric synthesis from prochiral precursors, where applicable. Finally, the last chapter’s section illustrates practical procedures. Notably, the presented biocatalytic methods can yield primary, secondary and tertiary amine products and often with excellent chemo-, regio- and stereoselectivity under mild reaction conditions.
asymmetric amination • amine resolution/deracemization • hydrolases • amine oxidases • transaminases • amine dehydrogenases • imine reductases • ammonia lyases • Pictet-Spenglerases • cytochromes P450
About the Book
This book presents an overview with a clear focus on practical aspects on biocatalysis. Reliable experimental information, useful purification methods and tips and tricks for the use of enzymes are included. A must-have for everyone who works or is going to work in this field.
Biocatalysis for Practitioners. Techniques, Reactions and Applications, (Ed. de Gonzalo, G.; Lavandera, I.), Wiley, Weinheim (Germany), 2021, Print: ISBN: 978-3-527-34683-7. e-book ISBN: 978-3-527-82445-8
Link to book: